Multiplex Real-Time PCR Method To Identify Shiga Toxin Genes stx1 and stx2 and Escherichia coli O157:H7/H(−) Serotype

A multiplex real-time PCR method to simultaneously detect the stx1 and stx2 genes of Shiga toxin-producing Escherichia coli and a unique conserved single-nucleotide polymorphism in the E. coli O157:H7/H(−) uidA gene has been developed. There is more than 98.6% sensitivity and 100% specificity for al...

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Detalhes bibliográficos
Main Authors: Jinneman, Karen C., Yoshitomi, Ken J., Weagant, Stephen D.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC201207/
https://ncbi.nlm.nih.gov/pubmed/14532101
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.10.6327-6333.2003
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