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Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle

A multiplex loop-mediated isothermal amplification (mLAMP) assay was developed for simultaneous detection of the stx1 and stx2 genes and applied for detection of shiga toxin-producing Escherichia coli (STEC) in cattle farm samples. Two target genes were distinguished based on T(m) values of 85.03 ±...

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Detalhes bibliográficos
Main Authors: Dong, Hee-Jin, Cho, Ae-Ri, Hahn, Tae-Wook, Cho, Seongbeom
Formato: Artigo
Idioma:Inglês
Publicado em: The Korean Society of Veterinary Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4087235/
https://ncbi.nlm.nih.gov/pubmed/24675834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4142/jvs.2014.15.2.317
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