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Baculovirus-mediated expression, purification, and characterization of a fully activated catalytic kinase domain construct of the 70 kDa 40S ribosomal protein S6 kinase-1 αII isoform (S6K1αII)

S6K1αII is a member of the AGC subfamily of serine-threonine protein kinases, whereby catalytic activation requires dual phosphorylation of critical residues in the conserved T-loop (T229) and hydrophobic motif (HM; T389) regions of its catalytic kinase domain [S6K1αII(ΔAID); deletion of C-terminal...

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Detalhes bibliográficos
Main Authors: Keshwani, Malik M., Ross, Duncan B., Ragan, Timothy J., Harris, Thomas K.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2275805/
https://ncbi.nlm.nih.gov/pubmed/18160308
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2007.11.003
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