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Cell-free cloning of highly expanded CTG repeats by amplification of dimerized expanded repeats
We describe conditions for producing uninterrupted expanded CTG repeats consisting of up to 2000 repeats using ϕ29 DNA polymerase. Previously, generation of such repeats was hindered by CTG repeat instability in plasmid vectors maintained in Escherichia coli and poor in vitro ligation of CTG repeat...
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| Main Authors: | , |
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| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
Oxford University Press
2008
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2275075/ https://ncbi.nlm.nih.gov/pubmed/18263610 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn025 |
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