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Site-specific Variations in RNA Folding Thermodynamics Visualized by 2-Aminopurine Fluorescence

The fluorescent base analogue 2-aminopurine (2-AP) is commonly used to study specific conformational and protein-binding events involving nucleic acids. Here, combinations of steady-state and time-resolved fluorescence spectroscopy of 2-AP were employed to monitor conformational transitions within a...

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Detalhes bibliográficos
Main Authors: Ballin, Jeff D., Bharill, Shashank, Fialcowitz-White, Elizabeth J., Gryczynski, Ignacy, Gryczynski, Zygmunt, Wilson, Gerald M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2274907/
https://ncbi.nlm.nih.gov/pubmed/17997580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi7011977
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