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Quantitative analysis of multi-protein interactions using FRET: Application to the SUMO pathway
Protein–protein binding and signaling pathways are important fields of biomedical science. Here we report simple optical methods for the determination of the equilibrium binding constant K(d) of protein–protein interactions as well as quantitative studies of biochemical cascades. The techniques are...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2271167/ https://ncbi.nlm.nih.gov/pubmed/18359863 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.073369608 |
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