Quantitative analysis of multi-protein interactions using FRET: Application to the SUMO pathway

Protein–protein binding and signaling pathways are important fields of biomedical science. Here we report simple optical methods for the determination of the equilibrium binding constant K(d) of protein–protein interactions as well as quantitative studies of biochemical cascades. The techniques are...

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Bibliographic Details
Main Authors: Martin, Sarah F., Tatham, Michael H., Hay, Ronald T., Samuel, Ifor D.W.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2008
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2271167/
https://ncbi.nlm.nih.gov/pubmed/18359863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.073369608
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