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A chemical method for fast and sensitive detection of DNA synthesis in vivo
We have developed a method to detect DNA synthesis in proliferating cells, based on the incorporation of 5-ethynyl-2′-deoxyuridine (EdU) and its subsequent detection by a fluorescent azide through a Cu(I)-catalyzed [3 + 2] cycloaddition reaction (“click” chemistry). Detection of the EdU label is hig...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
2008
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2268151/ https://ncbi.nlm.nih.gov/pubmed/18272492 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0712168105 |
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