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Exploring RNA transcription and turnover in vivo by using click chemistry
We describe a chemical method to detect RNA synthesis in cells, based on the biosynthetic incorporation of the uridine analog 5-ethynyluridine (EU) into newly transcribed RNA, on average once every 35 uridine residues in total RNA. EU-labeled cellular RNA is detected quickly and with high sensitivit...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2572917/ https://ncbi.nlm.nih.gov/pubmed/18840688 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0808480105 |
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