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Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences

A method of inhibiting the expression of particular genes by using external guide sequences (EGSs) has been improved in its rapidity and specificity. Random EGSs that have 14-nt random sequences are used in the selection procedure for an EGS that attacks the mRNA for a gene in a particular location....

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Détails bibliographiques
Auteurs principaux: Lundblad, Eirik W., Xiao, Gaoping, Ko, Jae-hyeong, Altman, Sidney
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 2008
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2268140/
https://ncbi.nlm.nih.gov/pubmed/18263737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0711977105
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