Isolation of a myoglobin molten globule by selective cobalt(III)-induced unfolding

Reaction of the Schiff-base complex [Co(acetylacetonate-ethylenediimine)(NH(3))(2)](+) with metmyoglobin at pH 6.5 yields a partially folded protein containing six Co(III) complexes. Although half of its α-helical secondary structure is retained, absorption and CD spectra indicate that the tertiary...

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Hauptverfasser: Blum, Ofer, Haiek, Abed, Cwikel, Dory, Dori, Zvi, Meade, Thomas J., Gray, Harry B.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The National Academy of Sciences 1998
Schlagworte:
Biological Sciences
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC22589/
https://ncbi.nlm.nih.gov/pubmed/9618468
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