Suppressing Posttranslational Gluconoylation of Heterologous Proteins by Metabolic Engineering of Escherichia coli

Minimization of chemical modifications during the production of proteins for pharmaceutical and medical applications is of fundamental and practical importance. The gluconoylation of heterologously expressed protein which is observed in Escherichia coli BL21(DE3) constitutes one such undesired postt...

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Autori principali: Aon, Juan C., Caimi, Richard J., Taylor, Alexander H., Lu, Quinn, Oluboyede, Femi, Dally, Jennifer, Kessler, Michelle D., Kerrigan, John J., Lewis, Tia S., Wysocki, Lisa A., Patel, Pramatesh S.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 2008
Soggetti:
Physiology and Biotechnology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2258596/
https://ncbi.nlm.nih.gov/pubmed/18083862
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01790-07
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