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Destabilizing Mutations Alter the Hydrogen Exchange Mechanism in Ribonuclease A

The effect of strongly destabilizing mutations, I106A and V108G of Ribonuclease A (RNase A), on its structure and stability has been determined by NMR. The solution structures of these variants are essentially equivalent to RNase A. The exchange rates of the most protected amide protons in RNase A (...

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Auteurs principaux: Bruix, Marta, Ribó, Marc, Benito, Antoni, Laurents, Douglas V., Rico, Manuel, Vilanova, Maria
Format: Artigo
Langue:Inglês
Publié: The Biophysical Society 2008
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2257908/
https://ncbi.nlm.nih.gov/pubmed/18192347
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.122952
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