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Conversion of 5-aminolevulinate synthase into a more active enzyme by linking the two subunits: Spectroscopic and kinetic properties

The two active sites of dimeric 5-aminolevulinate synthase (ALAS), a pyridoxal 5′-phosphate (PLP)-dependent enzyme, are located on the subunit interface with contribution of essential amino acids from each subunit. Linking the two subunits into a single polypeptide chain dimer (2XALAS) yielded an en...

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Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Zhang, Junshun, Cheltsov, Anton V., Ferreira, Gloria C.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Cold Spring Harbor Laboratory Press 2005
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC2253255/
https://ncbi.nlm.nih.gov/pubmed/15840827
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.041258305
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