Initial synthesis and characterization of an immobilized heat shock protein 90 column for online determination of binding affinities

Heat shock protein 90α (Hsp90α) was immobilized on aminopropyl silica via the N-terminus to create the Hsp90α(NT)-column or C-terminus to create the Hsp90α(CT)-column. Binding to the exposed C-terminus on the Hsp90α(NT)-column was characterized using frontal chromatography and C-terminus ligands cou...

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Hlavní autoři: Marszałł, Michał P., Moaddel, Ruin, Jozwiak, Krzysztof, Bernier, Michel, Wainer, Irving W.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2007
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2251629/
https://ncbi.nlm.nih.gov/pubmed/18047824
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.11.001
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