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Initial synthesis and characterization of an immobilized heat shock protein 90 column for online determination of binding affinities
Heat shock protein 90α (Hsp90α) was immobilized on aminopropyl silica via the N-terminus to create the Hsp90α(NT)-column or C-terminus to create the Hsp90α(CT)-column. Binding to the exposed C-terminus on the Hsp90α(NT)-column was characterized using frontal chromatography and C-terminus ligands cou...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2007
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2251629/ https://ncbi.nlm.nih.gov/pubmed/18047824 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.11.001 |
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