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The preparation and development of cellular membrane affinity chromatography columns

Cellular membrane affinity chromatography is a technique that is based on the immobilization of a target trans-membrane protein onto a stationary phase. The target protein is isolated by homogenization and solubilization of a source (e.g., cell line) followed by immobilization on either the immobili...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Moaddel, Ruin, Wainer, Irving W
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4605383/
https://ncbi.nlm.nih.gov/pubmed/19180089
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.225
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