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Enhancement of coenzyme binding by a single point mutation at the coenzyme binding domain of E. coli lactaldehyde dehydrogenase
Phenylacetaldehyde dehydrogenase (PAD) and lactaldehyde dehydrogenase (ALD) share some structural and kinetic properties. One difference is that PAD can use NAD(+) and NADP(+), whereas ALD only uses NAD(+). An acidic residue has been involved in the exclusion of NADP(+) from the active site in pyrid...
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| Main Author: | |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Cold Spring Harbor Laboratory Press
2008
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2248313/ https://ncbi.nlm.nih.gov/pubmed/18218709 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.073277108 |
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