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Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning
We have developed an efficient strategy that combines immunoglobulin (Ig) gene repertoire analysis and Ig reactivity profiling at the single cell level. Based on surface marker expression individual cells at different stages of human B cell development are isolated by fluorescence-activated cell sor...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2243222/ https://ncbi.nlm.nih.gov/pubmed/17996249 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2007.09.017 |
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