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Purification, crystallization and preliminary X-ray analysis of Mycobacterium tuberculosis folylpolyglutamate synthase (MtbFPGS)

The gene encoding Mycobacterium tuberculosis FPGS (MtbFPGS; Rv2447c) has been cloned and the protein (51 kDa) expressed in Escherichia coli. The purified protein was crystallized either by the batch method in the presence of adenosine diphosphate (ADP) and CoCl(2) or by vapour diffusion in the prese...

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Detalhes bibliográficos
Main Authors: Young, P. G., Smith, C. A., Sun, X., Baker, E. N., Metcalf, P.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2243083/
https://ncbi.nlm.nih.gov/pubmed/16754987
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106017180
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