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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of Rv2827c from Mycobacterium tuberculosis

The hypothetical protein Rv2827c from Mycobacterium tuberculosis was cloned and heterologously expressed in Escherichia coli. It was purified using affinity and size-exclusion chromatographic techniques and then crystallized. Preliminary X-ray diffraction data analysis suggests the presence of two t...

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Detalhes bibliográficos
Main Authors: Janowski, Robert, Nasser Eddine, Ali, Kaufmann, Stefan H. E., Weiss, Manfred S.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242928/
https://ncbi.nlm.nih.gov/pubmed/16880548
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106024213
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