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Manipulating and understanding antibiotic production in Streptomyces coelicolor A3(2) with decoy oligonucleotides
We have adapted and extended the decoy oligonucleotide technique for use in prokaryotes. To identify cis-acting regulatory elements within a promoter, we developed a DNase I/T7 exonuclease footprinting technique and applied it to actII-orf4 from Streptomyces coelicolor A3(2), which encodes the pathw...
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| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
2008
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2242687/ https://ncbi.nlm.nih.gov/pubmed/18187578 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0710724105 |
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