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Rapid-Equilibrium Rate Equations for the Enzymatic Catalysis of A + B = P + Q over a Range of pH†
This article shows how pKs for the enzymatic site and enzyme-substrate complexes can be obtained from kinetic experiments on the reaction A + B = P + Q, with and without the consumption of hydrogen ions. The rapid-equilibrium rate equation makes it possible to obtain the pKs and chemical equilibrium...
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2239239/ https://ncbi.nlm.nih.gov/pubmed/18061334 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpc.2007.10.015 |
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