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An Intermediate State of the γ-Aminobutyric Acid Transporter Gat1 Revealed by Simultaneous Voltage Clamp and Fluorescence

The rat γ-aminobutyric acid transporter GAT1 expressed in Xenopus oocytes was labeled at Cys74, and at one or more other sites, by tetramethylrhodamine-5-maleimide, without significantly altering GAT1 function. Voltage-jump relaxation analysis showed that fluorescence increased slightly and monotoni...

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Autori principali: Li, Ming, Farley, Robert A., Lester, Henry A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Rockefeller University Press 2000
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2233755/
https://ncbi.nlm.nih.gov/pubmed/10736315
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