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An Intermediate State of the γ-Aminobutyric Acid Transporter Gat1 Revealed by Simultaneous Voltage Clamp and Fluorescence
The rat γ-aminobutyric acid transporter GAT1 expressed in Xenopus oocytes was labeled at Cys74, and at one or more other sites, by tetramethylrhodamine-5-maleimide, without significantly altering GAT1 function. Voltage-jump relaxation analysis showed that fluorescence increased slightly and monotoni...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Rockefeller University Press
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2233755/ https://ncbi.nlm.nih.gov/pubmed/10736315 |
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