Purification, crystallization and preliminary X-ray diffraction studies of UDP-N-acetylglucosamine pyrophosphorylase from Candida albicans

UDP-N-acetylglucosamine pyrophosphorylase (UAP) is an essential enzyme in the synthesis of UDP-N-acetylglucosamine. UAP from Candida albicans was purified and crystallized by the sitting-drop vapour-diffusion method. The crystals of the substrate and product complexes both diffract X-rays to beyond...

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Detalhes bibliográficos
Main Authors: Maruyama, Daisuke, Nishitani, Yuichi, Nonaka, Tsuyoshi, Kita, Akiko, Fukami, Takaaki A., Mio, Toshiyuki, Yamada-Okabe, Hisafumi, Yamada-Okabe, Toshiko, Miki, Kunio
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2225379/
https://ncbi.nlm.nih.gov/pubmed/17142897
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106044186
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