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Purification, crystallization and preliminary X-ray crystallographic analysis of the outer membrane lipoprotein NlpE from Escherichia coli

The outer membrane lipoprotein NlpE functions in stress response by activating the Cpx signal transduction pathway. The nonlipidated Cys1Ala mutant of NlpE with a C-terminal His tag from Escherichia coli was constructed, overexpressed and purified. Crystals of NlpE were grown in two distinct forms b...

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Main Authors: Hirano, Yu, Hossain, Md. Motarab, Takeda, Kazuki, Tokuda, Hajime, Miki, Kunio
Formato: Artigo
Idioma:Inglês
Publicado: International Union of Crystallography 2006
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2225377/
https://ncbi.nlm.nih.gov/pubmed/17142903
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106045313
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