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Purification, crystallization and preliminary X-ray crystallographic analysis of the outer membrane lipoprotein NlpE from Escherichia coli

The outer membrane lipoprotein NlpE functions in stress response by activating the Cpx signal transduction pathway. The nonlipidated Cys1Ala mutant of NlpE with a C-terminal His tag from Escherichia coli was constructed, overexpressed and purified. Crystals of NlpE were grown in two distinct forms b...

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Bibliografiske detaljer
Main Authors: Hirano, Yu, Hossain, Md. Motarab, Takeda, Kazuki, Tokuda, Hajime, Miki, Kunio
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2225377/
https://ncbi.nlm.nih.gov/pubmed/17142903
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106045313
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