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Crystallization and X-ray diffraction analysis of 6-­aminohexanoate-cyclic-dimer hydrolase from Arthrobacter sp. KI72

6-Aminohexanoate-cyclic-dimer hydrolase (EI) from Arthrobacter sp. KI72 was expressed in Escherichia coli and purified by anion-exchange chromatography. EI was crystallized by the sitting-drop vapour-diffusion method with sodium citrate as precipitant in imidazole buffer pH 8.0. The crystal is hexag...

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Detalhes bibliográficos
Main Authors: Yasuhira, Kengo, Uedo, Yuki, Shibata, Naoki, Negoro, Seiji, Takeo, Masahiro, Higuchi, Yoshiki
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2225356/
https://ncbi.nlm.nih.gov/pubmed/17142898
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106045076
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