Crystallization and X-ray diffraction analysis of nylon-oligomer hydrolase (NylC) from Agromyces sp. KY5R

6-Aminohexanoate-oligomer hydrolase (NylC) from Agromyces sp. KY5R was expressed in Escherichia coli JM109 and purified by ammonium sulfate fractionation, anion-exchange column chromatography and gel-filtration chromatography. NylC was crystallized by the sitting-drop vapour-diffusion method with so...

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Detalhes bibliográficos
Main Authors: Yasuhira, Kengo, Shibata, Naoki, Tanaka, Yasuhito, Kumagai, Naoya, Tanaka, Yusuke, Nagai, Keisuke, Kato, Dai-ichiro, Takeo, Masahiro, Negoro, Seiji, Higuchi, Yoshiki
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2011
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3151121/
https://ncbi.nlm.nih.gov/pubmed/21821888
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111022858
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