Crystallization and X-ray diffraction analysis of nylon hydrolase (NylC) from Arthrobacter sp. KI72

Nylon hydrolase (NylC) encoded by Arthrobacter plasmid pOAD2 (NylC(p2)) was expressed in Escherichia coli JM109 and purified by ammonium sulfate fractionation, anion-exchange column chromatography and gel-filtration chromatography. NylC(p2) was crystallized by the sitting-drop vapour-diffusion metho...

Popoln opis

Shranjeno v:
Bibliografske podrobnosti
Main Authors: Nagai, Keisuke, Yasuhira, Kengo, Tanaka, Yusuke, Kato, Dai-ichiro, Takeo, Masahiro, Higuchi, Yoshiki, Negoro, Seiji, Shibata, Naoki
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2013
Teme:
Crystallization Communications
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3792678/
https://ncbi.nlm.nih.gov/pubmed/24100570
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113024263
Oznake: Označite
Brez oznak, prvi označite!

Podobne knjige/članki