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Single-molecule tracking of mRNA exiting from RNA polymerase II

Single-pair fluorescence resonance energy transfer was used to track RNA exiting from RNA polymerase II (Pol II) in elongation complexes. Measuring the distance between the RNA 5′ end and three known locations within the elongation complex allows us determine its position by means of triangulation....

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Andrecka, Joanna, Lewis, Robert, Brückner, Florian, Lehmann, Elisabeth, Cramer, Patrick, Michaelis, Jens
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2224174/
https://ncbi.nlm.nih.gov/pubmed/18162559
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0703815105
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