In vivo packaging of cosmids in transposon-mediated mutagenesis.

A technique was developed that permits the analysis of large regions of DNA by transposition mutagenesis. Large fragments of the pTiA6NC plasmid were cloned into the broad host range cosmid pHK17 and subjected to transposition mutagenesis by Tn3. Cosmids containing Tn3 insertions were selected by in...

ver descrição completa

Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	White, F F, Klee, H J, Nester, E W
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1983
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC221737/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6296041/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.153.2.1075-1078.1983
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!