In vivo packaging of cosmids in transposon-mediated mutagenesis.
A technique was developed that permits the analysis of large regions of DNA by transposition mutagenesis. Large fragments of the pTiA6NC plasmid were cloned into the broad host range cosmid pHK17 and subjected to transposition mutagenesis by Tn3. Cosmids containing Tn3 insertions were selected by in...
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| Pubblicato in: | J Bacteriol |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology (ASM)
1983
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC221737/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6296041/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.153.2.1075-1078.1983 |
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