Conformational change of the methionine 20 loop of Escherichia coli dihydrofolate reductase modulates pK(a) of the bound dihydrofolate

We evaluate the pK(a) of dihydrofolate (H(2)F) at the N(5) position in three ternary complexes with Escherichia coli dihydrofolate reductase (ecDHFR), namely ecDHFR(NADP(+):H(2)F) in the closed form (1), and the Michaelis complexes ecDHFR(NADPH:H(2)F) in the closed (2) and occluded (3) forms, by per...

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Hauptverfasser: Khavrutskii, Ilja V., Price, Daniel J., Lee, Jinhyuk, Brooks, Charles L.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2007
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2206655/
https://ncbi.nlm.nih.gov/pubmed/17473015
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.062724307
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