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Effect of Asp(69) and Arg(310) on the pK of His(68), a key catalytic residue of adenylosuccinate lyase
Adenylosuccinate lyase (ASL) of Bacillus subtilis contains three conserved histidines, His(68), His(89), and His(141), identified by affinity labeling and site-directed mutagenesis as critical to the intersubunit catalytic site. The pH-V(max) profile for wild-type ASL is bell-shaped (pK (1) = 6.74 a...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Cold Spring Harbor Laboratory Press
2007
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2203377/ https://ncbi.nlm.nih.gov/pubmed/17600142 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.072927207 |
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