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Effect of Asp(69) and Arg(310) on the pK of His(68), a key catalytic residue of adenylosuccinate lyase

Adenylosuccinate lyase (ASL) of Bacillus subtilis contains three conserved histidines, His(68), His(89), and His(141), identified by affinity labeling and site-directed mutagenesis as critical to the intersubunit catalytic site. The pH-V(max) profile for wild-type ASL is bell-shaped (pK (1) = 6.74 a...

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Hlavní autoři: Sivendran, Sharmila, Segall, Mark L., Rancy, Pumtiwitt C., Colman, Roberta F.
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2007
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2203377/
https://ncbi.nlm.nih.gov/pubmed/17600142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.072927207
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