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Bacillus subtilis DNA gyrase: purification of subunits and reconstitution of supercoiling activity.

DNA gyrase from Bacillus subtilis 168 was purified by affinity chromatography on novobiocin-Sepharose and shown to consist of two subunits, A and B, with molecular weights of 100,000 and 85,000, respectively. The B subunits, which contains novobiocin-sensitive. ATPase activity, could complement the...

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Dettagli Bibliografici
Pubblicato in:J Bacteriol
Autori principali: Orr, E, Staudenbauer, W L
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 1982
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC220277/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6282812/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.151.1.524-527.1982
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