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Bacillus subtilis DNA gyrase: purification of subunits and reconstitution of supercoiling activity.

DNA gyrase from Bacillus subtilis 168 was purified by affinity chromatography on novobiocin-Sepharose and shown to consist of two subunits, A and B, with molecular weights of 100,000 and 85,000, respectively. The B subunits, which contains novobiocin-sensitive. ATPase activity, could complement the...

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Detalhes bibliográficos
Main Authors: Orr, E, Staudenbauer, W L
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC220277/
https://ncbi.nlm.nih.gov/pubmed/6282812
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