Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds

A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris–HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature o...

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Detalhes bibliográficos
Main Authors: Cavada, Benildo S., Marinho, Emmanuel S., Souza, Emmanuel P., Benevides, Raquel G., Delatorre, Plínio, Souza, Luis A. G., Nascimento, Kyria S., Sampaio, Alexandre H., Moreno, Frederico B. M. B., Rustiguel, Joane K. R., Canduri, Fernanda, de Azevedo, Walter F., Debray, Henri
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2197170/
https://ncbi.nlm.nih.gov/pubmed/16511310
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910600371X
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