Standardized determination of real-time PCR efficiency from a single reaction set-up

We propose a computing method for the estimation of real-time PCR amplification efficiency. It is based on a statistic delimitation of the beginning of exponentially behaving observations in real-time PCR kinetics. PCR ground fluorescence phase, non-exponential and plateau phase were excluded from t...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Tichopad, Ales, Dilger, Michael, Schwarz, Gerhard, Pfaffl, Michael W.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2003
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC219490/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14530455/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng122
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