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Standardized determination of real-time PCR efficiency from a single reaction set-up
We propose a computing method for the estimation of real-time PCR amplification efficiency. It is based on a statistic delimitation of the beginning of exponentially behaving observations in real-time PCR kinetics. PCR ground fluorescence phase, non-exponential and plateau phase were excluded from t...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC219490/ https://ncbi.nlm.nih.gov/pubmed/14530455 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng122 |
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