Plaque color method for rapid isolation of novel recA mutants of Escherichia coli K-12: new classes of protease-constitutive recA mutants.

As a prerequisite to mutational analysis of functional sites on the RecA protein of Escherichia coli, a method was developed for rapid isolation of recA mutants with altered RecA protease function. The method involves plating mutagenized lambda recA+ cI ind on strains deleted for recA and containing...

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Bibliographic Details
Main Authors: Tessman, E S, Peterson, P
Format: Artigo
Language:Inglês
Published: 1985
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC219175/
https://ncbi.nlm.nih.gov/pubmed/3160686
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