Plaque color method for rapid isolation of novel recA mutants of Escherichia coli K-12: new classes of protease-constitutive recA mutants.
As a prerequisite to mutational analysis of functional sites on the RecA protein of Escherichia coli, a method was developed for rapid isolation of recA mutants with altered RecA protease function. The method involves plating mutagenized lambda recA+ cI ind on strains deleted for recA and containing...
Shranjeno v:
| izdano v: | J Bacteriol |
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| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology (ASM)
1985
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC219175/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3160686/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.163.2.677-687.1985 |
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