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Plaque color method for rapid isolation of novel recA mutants of Escherichia coli K-12: new classes of protease-constitutive recA mutants.

As a prerequisite to mutational analysis of functional sites on the RecA protein of Escherichia coli, a method was developed for rapid isolation of recA mutants with altered RecA protease function. The method involves plating mutagenized lambda recA+ cI ind on strains deleted for recA and containing...

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書目詳細資料
發表在:J Bacteriol
Principais autores: Tessman, E S, Peterson, P
格式: Artigo
語言:Inglês
出版: American Society for Microbiology (ASM) 1985
主題:
在線閱讀:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC219175/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3160686/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.163.2.677-687.1985
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