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DEVELOPING LIMITED PROTEOLYSIS AND MASS SPECTROMETRY FOR THE CHARACTERIZATION OF RIBOSOME TOPOGRAPHY
An approach that combines limited proteolysis and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been developed to probe protease-accessible sites of ribosomal proteins from intact ribosomes. Escherichia coli and Thermus thermophilus 70S ribosomes were subjected to limi...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2190778/ https://ncbi.nlm.nih.gov/pubmed/17521915 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jasms.2007.03.028 |
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