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DEVELOPING LIMITED PROTEOLYSIS AND MASS SPECTROMETRY FOR THE CHARACTERIZATION OF RIBOSOME TOPOGRAPHY

An approach that combines limited proteolysis and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been developed to probe protease-accessible sites of ribosomal proteins from intact ribosomes. Escherichia coli and Thermus thermophilus 70S ribosomes were subjected to limi...

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Detalhes bibliográficos
Main Authors: Suh, Moo-Jin, Pourshahian, Soheil, Limbach, Patrick A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2190778/
https://ncbi.nlm.nih.gov/pubmed/17521915
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jasms.2007.03.028
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