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A comparison of the folding characteristics of free and ribosome-tethered polypeptide chains using limited proteolysis and mass spectrometry

The kinetics and thermodynamics of protein folding are commonly studied in vitro by denaturing/renaturing intact protein sequences. How these folding mechanisms relate to de novo folding that occurs as the nascent polypeptide emerges from the ribosome is much less well understood. Here, we have empl...

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Detalhes bibliográficos
Publicado no:Protein Sci
Main Authors: Rajabi, Khadijeh, Reuther, Julia, Deuerling, Elke, Radford, Sheena E, Ashcroft, Alison E
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley & Sons, Ltd 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4534179/
https://ncbi.nlm.nih.gov/pubmed/25970093
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2702
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