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Clonal selection and in vivo quantitation of protein interactions with protein-fragment complementation assays

Two strategies are described for detecting constitutive or induced protein–protein interactions in intact mammalian cells; these strategies are based on oligomerization domain-assisted complementation of rationally designed fragments of the murine enzyme dihydrofolate reductase (DHFR; EC 1.5.1.3). W...

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Bibliografiske detaljer
Main Authors: Remy, Ingrid, Michnick, Stephen W.
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences 1999
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC21870/
https://ncbi.nlm.nih.gov/pubmed/10318894
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