Clonal selection and in vivo quantitation of protein interactions with protein-fragment complementation assays
Two strategies are described for detecting constitutive or induced protein–protein interactions in intact mammalian cells; these strategies are based on oligomerization domain-assisted complementation of rationally designed fragments of the murine enzyme dihydrofolate reductase (DHFR; EC 1.5.1.3). W...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1999
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC21870/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10318894/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.10.5394 |
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