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Isolation and Characterization of Protoplasts from Saccharomyces rouxii

Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that fo...

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Bibliografski detalji
Glavni autori: Arnold, Wilfred N., Garrison, Robert G.
Format: Artigo
Jezik:Inglês
Izdano: 1979
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC218323/
https://ncbi.nlm.nih.gov/pubmed/438120
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