Isolation and Characterization of Protoplasts from Saccharomyces rouxii
Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that fo...
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| Gepubliceerd in: | J Bacteriol |
|---|---|
| Hoofdauteurs: | , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
American Society for Microbiology (ASM)
1979
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC218323/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/438120/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.137.3.1386-1394.1979 |
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